Identificaciones de ksanderson

D.Malloch 16-08-22/07 Densely gregarious (many) on a dead log of Fagus grandifolia, in mature forest of Fagus grandifolia, Acer saccharum, Betula papyrifera and B. alleghaniensis

In herbarium, New Brunswick Museum (NBM)

Pileus convex-hemisphericalat first, expanding to only slightly more broadly so at maturity, without an umbo, striate at the margin, tacky, glabrous, bright orange (HSV40:70:100), 7-20 mm in diameter

Stipe equal, light yellow orange (HSV40:15-20:100), glabrous, moist, 22-50 x 2-3 mm

Lamellae light orange (HSV40:15:100), close, ascending-adnexed, red-marginate

Flesh light yellow in the pileus, concolorous with the surface tissues in the stipe the stipe, with a nondescript mushroom odour

Basidiospores white in spore print, mostly phaseoliform in profile, less frequently obovate, smooth, amyloid, 7.2-9.4 x 4.5-5.2 µm, Q = 1.54-2.06 (average[45]: 8.3±0.5 x 4.9±0.2 µm, Q = 1.71±-.09)

Basidia clavate, mostly 4-spored, less commonly 2-spored, with a basal clamp connection

Pileipellis of two layers, 1) a thick hyaline gelatinous lower layer and 2) a thin non-gelatinous cutis-like upper layer of smooth hyphae containing a yellow cytoplasmic pigment

Lamellar trama composted of a narrow parallel core and a thick gelatinous subhymenium

Pleurocystidia scattered, ventricose, often with a short finger-like apical extension, with a reddish orange cytoplasmic pigment, 29.9-62.8 x 8.3-15.3 µm, Q = 2.64-4.75 (average[24]: 45.2±8.0 x 12.0±2.0 µm, Q = 3.80±0.58)

Cheilocystidia forming a continuous sterile margin on the lamellae, similar to the pleurocystidia although less frequently with an apical extension, with a reddish orange cytoplasmic pigment, 34.8-44.9 x 7.9-14.8 µm, Q = 2.59-5.35 (average[10]: 39.8±3.4 x 10.7±1.9 µm, Q = 3.81±0.65)

Photos:

1. Basidiomata in the natural habitat
2. Scan of basidiomata
3. Cross section of the pileipellis in KOH + Congo Red
4. Cross section of the tip of a lamella in KOH + Congo Red showing the cheilocystidia
5. Cross section of a lamella in Windex showing two pleurocystidia
6. Pleurocystidia in KOH + Congo Red
7. Cheilocystidia in KOH + Congo Red
8. Basidiospores from spore print in Melzer's Solution

D.Malloch 15-08-22/09 Scattered to gregarious in leaf litter, in a mature forest of Fagus grandifolia, Acer saccharum, Betula papyrifera and B. alleghaniensis

In herbarium, New Brunswick Museum (NBM)

Pileus convex, smooth, dry, glabrous, bright pinkish red (HSV00:40:90), 50-75 mm in diameter

Stipe equal, fundamentally yellow (HSV50:30-40:100) but flushed throughout with the pinkish colours of the pileus, glabrous, dry, 78-95 x 17-20 mm

Pores yellow (HSV50:20:100), crowded, 2-2.4/mm, turning blue when damaged

Flesh yellow (HSV50:20:100), turning blue when cut, with a nondescript mushroom odour and taste

Basidiospores producing a very slight print on a microscope slide (discarded after microscopic examination), boletoid, smooth, not dextrinoid, 9.3-12.8 x 3.5-4.4 µm, Q = 2.66-3.54 (average[41]: 11.1±1.0 x 3.7±0.2 µm, Q = 2.98±0.22)

Hymenial cystidia abundant, ventricose to ventricose-rostrate, 27.9-53.8 x 5.2-11.2 µm, Q = 3.38-6.07 (average[26]: 36.9±6.4 x 8.4±1.2 µm, Q = 4.47±0.76)

Pileipellis a nearly vertical trichodermium at first, later becoming more lax and collapsed down, 200-350 µm deep, difficult at its lower levels to differentiate from the loosely parallel to interwoven tissues of the pileal trama

Photos:

1. Young basidiomata in the natural habitat
2. Older basidiomata in the natural habitat
3. Scan of basidiomata
4. Pileipellis in KOH + Congo Red
5. Hymenial cystidia in KOH + Congo Red
6. Basidiospores from spore print in Melzer's Solution

D.Malloch 21-08-22/05. Gregarious (2) on buried rotten wood, associated with Pinus strobus and Picea rubens in semimature forest of Picea rubens, Abies balsamea, Pinus strobus and Betula papyrifera

In herbarium, New Brunswick Museum (NBM)

Pileus convex, glabrous, dry, yellow brown (HSV40:20-30:90), 80 mm in diameter

Stipe tapering up, dry, glabrous below the reticulate apex, pale yellow (HSV50:05-10:100), 65-67 x 9-17 mm.

Pores pale pink (HSV00:05-10:100), adnexed, about 1.4/mm.

Flesh white or nearly so, with an indistinct mushroom odour, very bitter.

Basidiospores observed in a rather thin print, very pale orange pink (HSV25:05:100), boletoid, smooth, dextrinoid, 9.9-15.6 x 3.7-4.6 µm, Q = 2.50-3.62 (average[40]: 13.2±1.1 x 4.1±0.2 µm, Q = 3.22±0.24)

Cheilocystidia abundant at the tube mouths, ventricose-rostrate, with apex narrow and drawn out into a beak, with copious internal material that stains heavily in KOH+Congo red, 22.8-51.5 x 5.9-11.5 µm, Q = 2.98-4.91 (average[22]:36.0±7.7 x 8.8±1.5 µm, Q = 4.10±0.56).

Pleurocystidia abundant on the walls of the tubes, similar in shape to the cheilocystidia, mostly without internal material staining heavily in KOH + Congo Red, 23.2-60.0 x 8.6-16.7 µm, Q = 2.53-4.75 (average[25]: 43.9±7.7 x 12.7±2.1 µm, Q = 3.49±0.60)

Pileipellis an ascending to subascending ixotrichodermium, becoming more colllapsed in some parts, with hyphae having yellow-orange interior contents that are unaffected by water or Windex and exterior encrustations of similar material, with orange material apparently soluble in KOH and leaving the cells clear and yellow and without the orange exterior material, with many hyphae showing hyaline radially encrusted walls after KOH treatment

Hymenophoral trama bilateral

Photos:

1. Basidiomata in the natural habitat. Note the leopard slug (Limax maximus) between the two basidiomata!
2. Scan of basidiomata
3. Cross section of the pileipellis in Windex (left) followed by the introduction of KOH. Notice that the dark orange brown material in and around the hyphae have been dissolved in the KOH.
4. Cross section of a tube wall in KOH + Congo Red showing the bilateral trama
5. Pleurocystidia in KOH + Congo Red
6. Cheilocystidia in KOH + Congo Red
7. Basidiospores from spore print in Melzer's Solution. The spores are colorless when mounted in water but become dark red-brown (dextrinoid) in Melzer's.

D.Malloch 20-08-22/03. Gregarious (3) in humus, in medium-age forest of Pinus strobus, Abies balsamea, Picea rubens and Betula papyrifera

In herbarium, New Brunswick Museum (NBM)

Pileus conical to convex, with a large broad umbo, dry, densely scaly on the umbo, fibrillose toward the margin, dark brown (HSV20:50:40-50) on the umbo, more yellow brown (HSV30:20:90) toward the margin, 28-38 mm in diameter

Stipe equal, dry, fibrillose-scaly below the apex, yellow brown (HSV30:20:90), 45-67 x 4-6 mm.

Lamellae light brown (HSV30:10-15:90), adnexed, close.

Flesh pale olive (HSV45:10:70-80) in the pileus, light brown in the stipe, with a pronounced mushroom odour.

Basidiospores forming a deposit too thin to assign to colour, with prominent high nodules, usually with 5-6 nodules visible in dorsi-ventral view and 5 in profile, 8.4-12.2 x 4.8-8.2 µm, Q = 1.26-1.95 (average[52]: 9.9±0.82 x 6.5±0.63, Q = 1.52±0.16).

Basidia clavate, 4-spored, with a basal clamp connection

Cheilocystidia ventricose to lageniform, rarely with a capitulum, thin-walled, usually with apical crystals, often with elongated internal crystals, 40-80 x 13.0-26.8 µm.

Pleurocystidia ventricose to lageniform, thin-walled, usually with apical crystals, often with elongated internal crystals, 58-80 x 15.6-23.4 µm.

Photos:

1. Basidiomata in the natural habitat
2. Scan of basidiomata
3. Pleurocystidia in KOH + Congo Red
4. cheilocystidia in KOH + Congo Red
5. Basidiospores from lamella in KOH + Congo Red
6. Basidiospres from spore print in Melzer's Solution

D.Malloch 20-08-22/06. Solitary in soil and needle litter, associated with Pinus strobus in medium-age forest of Pinus strobus, Abies balsamea, Picea rubens and Betula papyrifera

In herbarium, New Brunswick Museum (NBM)

D.Malloch 21-08-22/07 Gregarious (2) in humus beside a path, associated with Picea rubens in semimature forest of Picea rubens, Abies balsamea, Pinus strobus and Betula papyrifera

In herbarium, New Brunswick Museum (NBM)

Pileus conical at first, not seen when fully expanded, glabrous, sticky, white, 40-62 mm in diameter

Stipe tapering up gradually, with a large subspherical bulb, dry, glabrous to finely diffracted-scaly, white, annulate, volvate, 35-50 x 6-9 mm.

Lamellae white, free, close

Flesh white, with an indistinct to slightly unpleasant mushroom odour.

Basidiospores white in spore print, broadly obovate to subspherical in profile, smooth, distinctly amyloid, 8.6-13.5(15.8) x 7.7-11.8 (14.1) µm, Q = 1.00-1.17 (average[41]: 10.5±1.28 x 9.6±1.10 µm, Q = 1.09±0.04).

Basidia clavate, both two- and four-spored but with four-spored more abundant

Pileipellis a thick ixocutis, with subpellis a compact cutis of narrow hyphae

Pileal trama irregularly and loosely interwoven

Lamellar trama bilateral, with a narrow parallel core and a broad and loose subhymenium

Photos:

1. Basidiomata in the natural habitat
2. Scan of basidiomata
3. Cross section of pileipellis in KOH + Congo Red
4. Cross section of lamella in KOH + Congo Red
5. Cross section of lamella in KOH + Congo Red showing details of subhymenium
6. Cross section of the volva in KOH + Congo Red
7. Basidia in KOH + Congo Red. Note both 2- and 4-spored basidia
8. Basidiospores from spore print in Melzer's Solution