Mag. 400x
The size range recorded for the current specimens was 70-130µ (see 2nd image, a composite of 9 specimens). The largest one is shown in the first photograph. All have lightly granulated cell walls and tapered, blunt apices (like inward turned Dixie cup-shapes). G. brebissonii has been previously observed in this slow-moving, rocky, vernal stream; in 2021: https://www.inaturalist.org/observations/72575550). Both geraldojpr and roman_romanov participated in determining the ID and describing its growth characteristics. geraldojpr further refined the ID to Gonatozygon brebissonii var. vulgaris.
What amazes me is that this area is bone-dry during the summer months. Yet algal taxa can be counted on to reappear every Spring... hiding in pockets of moisture? by producing long-lived, durable zygospores? All without roots or an anchor of some kind. Too cool!
Mag. 400x
I'm not certain about the ID, but will check with geraldojpr and roman_romanov. Most fascinating about this specimen is that one semi-cell is 3-radiate (3 lobes) and the other is 6-radiate. An intermediate polymorph! Will its progeny be 3- or 6 radiate or a hybrid of both? Interesting! S. margaritaceum is noted to be "variable in the in the number of radial processes, , with 4-6 being the most often found" (https://www.outerhebridesalgae.uk/desmids/desmid-species.php?id=359) ... I could not find a reference noting 3-radiate S. margaritaceum. The first composite image shows the specimen in lateral view, then beginning to roll backwards. The second composite of 9 photos shows the same specimen making a ~180⁰ rotation about a slanted axis. (Panels 8 and 9 vary only in depth of field, not position of the specimen.) Too cool!
For more reference images of S. margaritaceum see:
Mag. 200x
Two daughter cells, still attached, of a Closterium sp,. More robustly proportioned than C. kuetzingii (fatter, but with similarly tapering and gently curved apices), a diagnosis of Closterium rostratum seems clear, as seen here http://www.digicodes.info/Closterium_rostratum.html. In the 2nd image, "N" marks the nuclear region (which will eventually become centrally located as each daughter cell matures). Also marked are the 2 chloroplasts normally found in Closterium (one per semi-cell on each side of the nuclear region). I could not find an image of a dividing C. rostratum, but a dividing C. venus, as seen here, https://www.photomacrography.net/forum/viewtopic.php?t=25375, serves as a model.
Magnification of photos: 400×, 400×, 400×
Habitat: filamentous green algae (mostly Mougeotia and Spirogyra) and some organic debris collected from a freshwater pond.
Photo taken with a Celestron PentaView Digital Microscope. According to their website, the FOV (i.e. the diagonal width) at 400× is 150 µm.
Magnification of photos: 200×, 200×, 400×, 400×, 600×, 600×
Habitat: filamentous green algae (mostly Mougeotia and Spirogyra) and some organic debris collected from a freshwater pond.
Photo taken with a Celestron PentaView Digital Microscope. According to their website, the FOV (i.e. the diagonal width) at 400× is 150 µm.